Nextonic Solutions is seeking a highly ambitious, adaptable, structured, and detail-oriented Biologist to join our vibrant team at the National Institutes of Health (NIH), National Institute of Allergy and Infectious Diseases (NIAID), Vaccine Research Center (VRC), Virus Persistence and Dynamics Section (VPDS)
-Prepare experimental reagents and supplies.
-Perform mammalian cell cultures.
-Prepare, provide and develop tonsilar organoid culture models.
-Develop isolating human cells from human blood.
-Develop multiparameter flow cytometric analysis to analyze lymphocyte subsets
-Prepare and design flow cytometry antibody panels to address new questions by flow cytometry.
-Prepare and develop intracellular staining techniques to measure cellular and viral protein expression.
-Prepare and promote the propagation of HIV-1 virus
-Develop HIV-1 viral genomic RNA measurements.
-Prepare molecular assays to detect and distinguish among different forms of the HIV-1 genome in virus-infected cells.
-Prepare p24 ELISA assays to measure HIV-1 replication.
-Prepare and develop single-cell sequencing methodologies.
-Interact with external scientists as part of research collaborations.
-Prepare internal and external presentations and research manuscripts.
Experience Required / Requirements
-Master’s degree in biology, Life Sciences or a related discipline.
-Ability to keep up to date with scientific developments relevant to the field and project.