Nextonic is seeking a highly ambitious, adaptable, structured, and detail-oriented Scientist to join our vibrant team at the National Institutes of Health (NIH), National Institute of Allergy and Infectious Diseases (NIAID), Vaccine Production Program located in Gaithersburg, MD.
- Advance vaccine and therapeutic protein candidates including monoclonal antibodies, recombinant proteins, and virus-like particles by developing stable cell lines for preclinical development programs.
- Independently culture mammalian cells in plates, shake flasks, and small-scale bioreactors with aseptic technique.
- Perform transfection, selection, cloning, and maintenance of cells expressing proteins of interest.
- Generate stable pools and perform transient protein production for supply of reagent-grade material.
- Prepare plasmid DNA using standard molecular cloning techniques.
- Participate in cell line development platform design, optimization, and cycle-time reduction studies including methods for clone selection, expansion, ranking, and characterization by reviewing recent literature and new technologies.
- Identify, propose, and execute process and procedural changes to improve process performance, robustness, productivity, and efficiency.
- Independently design, perform, and troubleshoot experiments and analyze data.
- Plan and execute cell line development projects from plasmid construction through top clone selection.
- Oversee maintenance and support for one or more pieces of laboratory instrumentation.
- Generate, maintain, and review accurate daily records of experiments and results in a laboratory notebook.
- Prepare and review experimental plans, proposals, reports, SOPs, and production records.
- Collect information, draft and verify data for CMC sections of pre-IND and IND documents to ensure successful registration of candidates.
- Review experiments in order to evaluate the progress of the research and ensure that experiments and documentation conform to established protocols.
- Work closely with other members of CLD team, upstream process development and downstream process development teams to improve workflow efficiency and overall process yield.
- Work with Federal Task Leader to manage project timelines and provide regular updates to project teams.
- Coordinate team meeting updates and presentations.
- Ph.D. in Biological Sciences with a minimum of 2 years of independent research experience in an academic or industrial setting.
- Hands-on experience with aseptic technique and mammalian cell culture along with supporting technologies for cell line engineering including cell counting, high-throughput transfection, single-cell cloning, flow cytometry, and automated small-scale bioreactor systems.
- Familiarity with molecular cloning methods, PCR, and agarose gel electrophoresis as well as supporting software for generating and editing plasmid maps.
- Ability to independently design experiments and work plans and manage project timelines for complex projects.
- Experience in preparing technical reports, standard operating procedures, and data presentations.